Privacy Protection Framework for Service-Oriented Architecture

Service-Oriented Architecture (SOA) is a computer systems design concept which aims to achieve reusability and integration in a distributed environment through the use of autonomous, loosely coupled, interoperable abstractions known as services. In order to interoperate, communication between services is very important due to their autonomous nature. This communication provides services with their functional strengths, but also creates the opportunity for the loss of privacy. In this thesis, a Privacy Protection Framework for Service-Oriented Architecture (PPFSOA) is proposed. In this framework, a Privacy Service (PS) is used in combination with privacy policies to create privacy contracts that outline what can and cannot be done with a consumer’s personally identifiable information (PII). The privacy policy consists of one-to-many privacy rules, with each rule created from a set of six privacy elements: collector, what, purpose, retention, recipient and trust. These elements were carefully selected from a set of Fair Information Practices (FIP) which has been used as the basis for privacy legislation around the world. The PS plays the role of negotiator, comparing privacy policies to create privacy contracts and resolving any conflicts that arise during this process. The PS acts as an intermediary between the service consumer and service provider, to establish an unbiased contract before the two parties begin sending PII. An examination of what current approaches to protecting privacy in an SOA environment is also presented. Finally, a proof of concept is shown which demonstrates the behaviour of a functioning PS in multiple scenario

Protecting Personal Private Information in Collaborative Environments

The ability to collaborate has always been vitally important to businesses and enterprises. With the availability of current networking and computing power, the creation of Collaborative Working Environments (CWEs) has allowed for this process to occur anytime over any geographical distance. Sharing information between individuals through collaborative environments creates new challenges in privacy protection for organizations and the members of organizations. This thesis confronts the problems when attempting to protect the personal private information of collaborating individuals. In this thesis, a privacy-by-policy approach is taken to addressing the issue of protecting private information within collaborative environments. A privacy-by-policy approach to privacy protection provides collaborating individuals with notice and choice surrounding their private information, in order to provide an individual with a level of control over how their information is to be used. To this end, a collaborative privacy architecture for providing privacy within a collaborative environment is presented. This architecture uses ontologies to express the static concept and relation definitions required for privacy and collaboration. The collaborative privacy architecture also contains a Collaborative Privacy Manager (CPM) service which handles changes in dynamic collaborative environments. The goals of this thesis are to provide privacy mechanisms for the non-client centric situation of collaborative working environments. This thesis also strives to provide privacy through technically enforceable and customizable privacy policies. To this end, individual collaborators are provided with access, modification rights, and transparency through the use of ontologies built into the architecture. Finally, individual collaborators are provided these privacy protections in a way that is easy to use and understand and use. A collaborative scenario as a test case is described to present how this architecture would benefit individuals and organizations when they are engaged in collaborative work. In this case study a university and hospital are engaged in collaborative research which involves the use of private information belonging to collaborators and patients from the hospital. This case study also highlights how different organizations can be under different sets of legislative guidelines and how these guidelines can be incorporated into the privacy architecture. Through this collaboration scenario an implementation of the collaborative privacy architecture is provided, along with results from semantic and privacy rule executions, and measurements of how actions carried out by the architecture perform under various conditions

Myxobacterial Predation:A Standardised Lawn Predation Assay Highlights Strains with Unusually Efficient Predatory Activity

Myxobacteria prey upon a broad range of microorganisms. Lawn assays are commonly used to quantify myxobacterial predation—myxobacterial suspensions are spotted onto prey lawns, and monitored via spot expansion. The diversity in motility behaviours of myxobacterial strains and differing assay protocols in myxobacteriology laboratories led us to develop a highly-specified assay, which was applied to 28 myxobacterial strains preying on seven phytopathogenic prey species. Generally, prey organisms showed no qualitative differences in their susceptibility/resistance to myxobacterial predation. For most myxobacteria, prey did not stimulate, and in ~50% of cases actively hindered colony expansion. Only ~25% of predator/prey strain combinations exhibited greater colony expansion than in the absence of nutrients. The activity of predatory strains against different prey correlated, implying effective predators may have relatively non-specific predation mechanisms (e.g., broad specificity proteases/lipases), but no correlation was observed between predatory activity and phylogeny. Predation on dead (but intact) or lysed prey cells gave greater colony expansion than on live prey. Occasional strains grew substantially faster on dead compared to lysed cells, or vice-versa. Such differences in accessing nutrients from live, dead and lysed cells indicates there are strain-specific differences in the efficiencies/machineries of prey killing and nutrient acquisition, which has important implications for the ecology of myxobacterial predators and their prey

Quantitative nucleotide level analysis of regulation of translation in response to depolarization of cultured neural cells

Studies on regulation of gene expression have contributed substantially to understanding mechanisms for the long-term activity-dependent alterations in neural connectivity that are thought to mediate learning and memory. Most of these studies, however, have focused on the regulation of mRNA transcription. Here, we utilized high-throughput sequencing coupled with ribosome footprinting to globally characterize the regulation of translation in primary mixed neuronal-glial cultures in response to sustained depolarization. We identified substantial and complex regulation of translation, with many transcripts demonstrating changes in ribosomal occupancy independent of transcriptional changes. We also examined sequence-based mechanisms that might regulate changes in translation in response to depolarization. We found that these are partially mediated by features in the mRNA sequence—notably upstream open reading frames and secondary structure in the 5′ untranslated region—both of which predict downregulation in response to depolarization. Translationally regulated transcripts are also more likely to be targets of FMRP and include genes implicated in autism in humans. Our findings support the idea that control of mRNA translation plays an important role in response to neural activity across the genome

Within-species variation in OMV cargo proteins:The Myxococcus xanthus OMV pan-proteome

Extracellular membrane vesicles are produced by all domains of life (bacteria, archaea and eukaryotes). Bacterial extracellular vesicles (outer membrane vesicles or OMVs) are produced by outer membrane blebbing, and contain proteins, nucleic acids, virulence factors, lipids and metabolites. OMV functions depend on their internal composition, therefore understanding the proteome of OMVs, and how it varies between organisms, is imperative. Here, we report a comparative proteomic profiling of OMVs from strains of Myxococcus xanthus, a predatory species of Gram-negative myxobacteria whose secretions include secondary metabolites and hydrolytic enzymes, thought to be involved in prey lysis. Ten strains were chosen for study, of which seven had genome sequences available. The remaining three strains were genome sequenced allowing definition of the core and accessory genes and genome-derived proteins found within the pan-genome and pan-proteome respectively. OMVs were isolated from each strain and proteins identified using mass spectrometry. The M. xanthus OMV pan-proteome was found to contain tens of ‘core’ and hundreds of ‘accessory’ proteins. Properties of the OMV pan-proteome were compared with those of the pan-proteome deduced from the M. xanthus pan-genome. On average, 80% of ‘core’ OMV proteins are encoded by genes of the core genome, yet the OMV proteomes of individual strains contain subsets of core genome-derived proteins which only partially overlap. In addition, the distribution of characteristics of vesicle proteins does not correlate with the genome-derived proteome characteristic distribution. We hypothesize that M. xanthus cells package a personalized subset of proteins whose availability is only partially dictated by the presence/absence of encoding genes within the genome

Two Weeks of Ischemic Conditioning Improves Walking Speed and Reduces Neuromuscular Fatigability in Chronic Stroke Survivors

This pilot study examined whether ischemic conditioning (IC), a noninvasive, cost-effective, and easy-to-administer intervention, could improve gait speed and paretic leg muscle function in stroke survivors. We hypothesized that 2 wk of IC training would increase self-selected walking speed, increase paretic muscle strength, and reduce neuromuscular fatigability in chronic stroke survivors. Twenty-two chronic stroke survivors received either IC or IC Sham on their paretic leg every other day for 2 wk (7 total sessions). IC involved 5-min bouts of ischemia, repeated five times, using a cuff inflated to 225 mmHg on the paretic thigh. For IC Sham, the cuff inflation pressure was 10 mmHg. Self-selected walking speed was assessed using the 10-m walk test, and paretic leg knee extensor strength and fatigability were assessed using a Biodex dynamometer. Self-selected walking speed increased in the IC group (0.86 ± 0.21 m/s pretest vs. 1.04 ± 0.22 m/s posttest, means ± SD; P\u3c 0.001) but not in the IC Sham group (0.92 ± 0.47 m/s pretest vs. 0.96 ± 0.46 m/s posttest; P= 0.25). Paretic leg maximum voluntary contractions were unchanged in both groups (103 ± 57 N·m pre-IC vs. 109 ± 65 N·m post-IC; 103 ± 59 N·m pre-IC Sham vs. 108 ± 67 N·m post-IC Sham; P = 0.81); however, participants in the IC group maintained a submaximal isometric contraction longer than participants in the IC Sham group (278 ± 163 s pre-IC vs. 496 ± 313 s post-IC, P = 0.004; 397 ± 203 s pre-IC Sham vs. 355 ± 195 s post-IC Sham; P = 0.46). The results from this pilot study thus indicate that IC training has the potential to improve walking speed and paretic muscle fatigue resistance poststroke